Hotspot

trnR-ACG-trnN-GUU

IRb · 110676-111292 · 617 bp

Back to Hemsleya

Pi 0.00513

MarkerSeek score 52.70

Primer available yes

Label trnR-ACG-trnN-GUU

Feature Metadata

label_name: trnR-ACG-trnN-GUU
feature_type: igs
region: IRb
length_bp: 617
variable_sites: 5
pi: 0.00513
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-ACG-trnN-GUU_p1	CAGAGGATTAGAGCACGTGG	CCGTTGTTCCTCAGTAGCTC	55.0	55.0	57.8	58.0	759 bp	1.0	78.8
trnR-ACG-trnN-GUU_p2	GAACAGATCCAGTGGAGACG	CCGTTGTTCCTCAGTAGCTC	55.0	55.0	57.7	58.0	796 bp	1.0	78.2
trnR-ACG-trnN-GUU_p3	CAGAGGATTAGAGCACGTGG	TGGTCGTAGGTTCGAATCCT	55.0	50.0	57.8	58.2	710 bp	1.0	77.6
trnR-ACG-trnN-GUU_p4	CAGAGGATTAGAGCACGTGG	CTGGTCGTAGGTTCGAATCC	55.0	55.0	57.8	57.8	711 bp	1.0	77.2
trnR-ACG-trnN-GUU_p5	GAACAGATCCAGTGGAGACG	TGGTCGTAGGTTCGAATCCT	55.0	50.0	57.7	58.2	747 bp	1.0	77.0