Hotspot

trnF-GAA-ndhJ

LSC · 51522-52249 · 728 bp

Back to Hemsleya

Pi 0.00229

MarkerSeek score 52.90

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 728
variable_sites: 3
pi: 0.00229
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	CCTGAAAGTTGGGTGGGATG	50.0	55.0	58.1	58.5	736-840 bp	1.0	80.3
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	CTGAAAGTTGGGTGGGATGG	50.0	55.0	58.1	58.5	735-839 bp	1.0	80.3
trnF-GAA-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	TAATCATCCCCGCCTAAAGC	50.0	50.0	58.1	57.4	767-871 bp	1.0	79.4
trnF-GAA-ndhJ_p4	CCTCGTGTCACCAGTTCAAA	AATGCCTGAAAGTTGGGTGG	50.0	50.0	58.1	58.7	740-844 bp	1.0	78.6
trnF-GAA-ndhJ_p5	TCGGGATAGCTCAGTTGGTA	CTGAAAGTTGGGTGGGATGG	50.0	55.0	57.6	58.5	773-877 bp	1.0	77.1