Hotspot

trnE-UUC-trnT-GGU

LSC · 33652-34566 · 915 bp

Back to Hemsleya

Pi 0.00437

MarkerSeek score 52.40

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 915
variable_sites: 8
pi: 0.00437
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	CGTAGTACTCTACCCCCAGG	GAACCGATGACTTACGCCTT	60.0	50.0	58.0	58.0	1048-1053 bp	1.0	80.8
trnE-UUC-trnT-GGU_p2	CGTAGTACTCTACCCCCAGG	CCATGGCGTTACTCTACCAC	60.0	55.0	58.0	58.1	1027-1032 bp	1.0	80.4
trnE-UUC-trnT-GGU_p3	CGTAGTACTCTACCCCCAGG	TGGCGTTACTCTACCACTGA	60.0	50.0	58.0	58.1	1024-1029 bp	1.0	80.2
trnE-UUC-trnT-GGU_p4	CGTAGTACTCTACCCCCAGG	CCGATGACTTACGCCTTACC	60.0	55.0	58.0	58.2	1045-1050 bp	1.0	79.7
trnE-UUC-trnT-GGU_p5	GCTGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	55.0	50.0	58.3	58.0	1013-1018 bp	1.0	78.9