Hotspot

psbM-trnD-GUC

LSC · 31976-33049 · 1074 bp

Back to Hemsleya

Pi 0.0678

MarkerSeek score 79.70

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 1074
variable_sites: 111
pi: 0.0678
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 79.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	AAAAAGGCGGTAGGAACGAG	TTCAATTGGTCAGAGCACCG	50.0	50.0	58.2	58.5	1203 bp	1.0	85.9
psbM-trnD-GUC_p2	GCGGTAGGAACGAGAATGAA	TTCAATTGGTCAGAGCACCG	50.0	50.0	57.7	58.5	1197 bp	1.0	85.2
psbM-trnD-GUC_p3	ACGAGAATGAACAGTGCAGT	TTCAATTGGTCAGAGCACCG	45.0	50.0	57.5	58.5	1188 bp	1.0	83.3
psbM-trnD-GUC_p4	AAAAAGGCGGTAGGAACGAG	GTTCAATTGGTCAGAGCACC	50.0	50.0	58.2	57.0	1204 bp	1.0	81.7
psbM-trnD-GUC_p5	AAGGCGGTAGGAACGAGAAT	TTCAATTGGTCAGAGCACCG	50.0	50.0	58.8	58.5	1200 bp	1.0	81.0