Hotspot

rpoB-trnC-GCA

LSC · 28548-29742 · 1195 bp

Back to Hemsleya

Pi 0.00432

MarkerSeek score 54.80

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1195
variable_sites: 10
pi: 0.00432
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CTCTCATTTCCACCCCCAAG	GCGTCTTTTGTTGATGAGGC	55.0	50.0	57.9	58.0	1302-1308 bp	1.0	79.6
rpoB-trnC-GCA_p2	CTCTCATTTCCACCCCCAAG	CCGGATTTGAACTGGGGAAA	55.0	50.0	57.9	58.1	1277-1283 bp	1.0	79.2
rpoB-trnC-GCA_p3	TCTCTCATTTCCACCCCCAA	GCGTCTTTTGTTGATGAGGC	50.0	50.0	58.2	58.0	1303-1309 bp	1.0	78.7
rpoB-trnC-GCA_p4	TTCTCTCATTTCCACCCCCA	GCGTCTTTTGTTGATGAGGC	50.0	50.0	58.2	58.0	1304-1310 bp	1.0	78.7
rpoB-trnC-GCA_p5	TCTCTCATTTCCACCCCCAA	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.2	58.1	1278-1284 bp	1.0	78.3