Hotspot

trnR-UCU-atpA

LSC · 11217-11584 · 368 bp

Back to Hemsleya

Pi 0.00996
MarkerSeek score 37.40
Primer available yes
Label trnR-UCU-atpA

Feature Metadata

label_name
trnR-UCU-atpA
feature_type
igs
region
LSC
length_bp
368
variable_sites
6
pi
0.00996
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
37.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-UCU-atpA_p1 AGCCTTCCAAGCTAACGATG TTCCACCAAGACATTCACCG 50.0 50.0 58.0 58.1 805-813 bp 1.0 80.3
trnR-UCU-atpA_p2 AGCCTTCCAAGCTAACGATG GCTTATCCGGGGGATGTTTT 50.0 50.0 58.0 57.9 1380-1388 bp 1.0 80.1
trnR-UCU-atpA_p3 AGCCTTCCAAGCTAACGATG GATCCCTCCAAACAAGCACA 50.0 50.0 58.0 58.1 1449-1457 bp 1.0 79.9
trnR-UCU-atpA_p4 AGCCTTCCAAGCTAACGATG TGATCCCTCCAAACAAGCAC 50.0 50.0 58.0 58.1 1450-1458 bp 1.0 79.9
trnR-UCU-atpA_p5 CCTAGCCTTCCAAGCTAACG GCTTATCCGGGGGATGTTTT 55.0 50.0 58.1 57.9 1383-1391 bp 1.0 79.9