Hotspot

rps16-trnQ-UUG

LSC · 6462-8199 · 1738 bp

Back to Hemsleya

Pi 0.00432

MarkerSeek score 60.10

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1738
variable_sites: 15
pi: 0.00432
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1806-1811 bp	1.0	79.2
rps16-trnQ-UUG_p2	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	1817-1822 bp	1.0	78.7
rps16-trnQ-UUG_p3	CGGTGGGTGTAAGAATCCAC	GAGGTTCGAATCCTTCCGTC	55.0	55.0	58.3	58.1	1843-1848 bp	1.0	78.2
rps16-trnQ-UUG_p4	AACGGTGGGTGTAAGAATCC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.5	58.1	1845-1850 bp	1.0	76.4
rps16-trnQ-UUG_p5	ACGGTGGGTGTAAGAATCCA	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.6	58.1	1844-1849 bp	1.0	75.1