primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	6462	8199	1806-1811	1.000000	79.2
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.2	58.1	0.0	16.9	0.280333	6462	8199	1817-1822	1.000000	78.7
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	CGGTGGGTGTAAGAATCCAC	GAGGTTCGAATCCTTCCGTC	20	20	55.0	55.0	58.3	58.1	0.0	16.9	0.341938	6462	8199	1843-1848	1.000000	78.2
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	AACGGTGGGTGTAAGAATCC	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.5	58.1	0.0	16.9	0.560843	6462	8199	1845-1850	1.000000	76.4
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	ACGGTGGGTGTAAGAATCCA	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.6	58.1	0.0	16.9	0.722000	6462	8199	1844-1849	1.000000	75.1
trnR-UCU-atpA_p1	trnR-UCU-atpA	1	AGCCTTCCAAGCTAACGATG	TTCCACCAAGACATTCACCG	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.138962	11217	11584	805-813	1.000000	80.3
trnR-UCU-atpA_p2	trnR-UCU-atpA	2	AGCCTTCCAAGCTAACGATG	GCTTATCCGGGGGATGTTTT	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.097204	11217	11584	1380-1388	1.000000	80.1
trnR-UCU-atpA_p3	trnR-UCU-atpA	3	AGCCTTCCAAGCTAACGATG	GATCCCTCCAAACAAGCACA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.120138	11217	11584	1449-1457	1.000000	79.9
trnR-UCU-atpA_p4	trnR-UCU-atpA	4	AGCCTTCCAAGCTAACGATG	TGATCCCTCCAAACAAGCAC	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.120138	11217	11584	1450-1458	1.000000	79.9
trnR-UCU-atpA_p5	trnR-UCU-atpA	5	CCTAGCCTTCCAAGCTAACG	GCTTATCCGGGGGATGTTTT	20	20	55.0	50.0	58.1	57.9	0.0	0.0	0.126040	11217	11584	1383-1391	1.000000	79.9
rpoB-trnC-GCA_p1	rpoB-trnC-GCA	1	CTCTCATTTCCACCCCCAAG	GCGTCTTTTGTTGATGAGGC	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.165686	28548	29742	1302-1308	1.000000	79.6
rpoB-trnC-GCA_p2	rpoB-trnC-GCA	2	CTCTCATTTCCACCCCCAAG	CCGGATTTGAACTGGGGAAA	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.216917	28548	29742	1277-1283	1.000000	79.2
rpoB-trnC-GCA_p3	rpoB-trnC-GCA	3	TCTCTCATTTCCACCCCCAA	GCGTCTTTTGTTGATGAGGC	20	20	50.0	50.0	58.2	58.0	0.0	0.0	0.276994	28548	29742	1303-1309	1.000000	78.7
rpoB-trnC-GCA_p4	rpoB-trnC-GCA	4	TTCTCTCATTTCCACCCCCA	GCGTCTTTTGTTGATGAGGC	20	20	50.0	50.0	58.2	58.0	0.0	0.0	0.276994	28548	29742	1304-1310	1.000000	78.7
rpoB-trnC-GCA_p5	rpoB-trnC-GCA	5	TCTCTCATTTCCACCCCCAA	CCGGATTTGAACTGGGGAAA	20	20	50.0	50.0	58.2	58.1	0.0	0.0	0.328225	28548	29742	1278-1284	1.000000	78.3
psbM-trnD-GUC_p1	psbM-trnD-GUC	1	AAAAAGGCGGTAGGAACGAG	TTCAATTGGTCAGAGCACCG	20	20	50.0	50.0	58.2	58.5	0.0	5.4	0.672731	31976	33049	1203-1203	1.000000	85.9
psbM-trnD-GUC_p2	psbM-trnD-GUC	2	GCGGTAGGAACGAGAATGAA	TTCAATTGGTCAGAGCACCG	20	20	50.0	50.0	57.7	58.5	0.0	5.4	0.766292	31976	33049	1197-1197	1.000000	85.2
psbM-trnD-GUC_p3	psbM-trnD-GUC	3	ACGAGAATGAACAGTGCAGT	TTCAATTGGTCAGAGCACCG	20	20	45.0	50.0	57.5	58.5	0.0	5.4	1.010598	31976	33049	1188-1188	1.000000	83.3
psbM-trnD-GUC_p4	psbM-trnD-GUC	4	AAAAAGGCGGTAGGAACGAG	GTTCAATTGGTCAGAGCACC	20	20	50.0	50.0	58.2	57.0	0.0	5.4	1.192884	31976	33049	1204-1204	1.000000	81.7
psbM-trnD-GUC_p5	psbM-trnD-GUC	5	AAGGCGGTAGGAACGAGAAT	TTCAATTGGTCAGAGCACCG	20	20	50.0	50.0	58.8	58.5	0.0	5.4	1.284903	31976	33049	1200-1200	1.000000	81.0
trnE-UUC-trnT-GGU_p1	trnE-UUC-trnT-GGU	1	CGTAGTACTCTACCCCCAGG	GAACCGATGACTTACGCCTT	20	20	60.0	50.0	58.0	58.0	0.0	0.0	0.027516	33652	34566	1048-1053	1.000000	80.8
trnE-UUC-trnT-GGU_p2	trnE-UUC-trnT-GGU	2	CGTAGTACTCTACCCCCAGG	CCATGGCGTTACTCTACCAC	20	20	60.0	55.0	58.0	58.1	0.0	0.0	0.085613	33652	34566	1027-1032	1.000000	80.4
trnE-UUC-trnT-GGU_p3	trnE-UUC-trnT-GGU	3	CGTAGTACTCTACCCCCAGG	TGGCGTTACTCTACCACTGA	20	20	60.0	50.0	58.0	58.1	0.0	0.0	0.107768	33652	34566	1024-1029	1.000000	80.2
trnE-UUC-trnT-GGU_p4	trnE-UUC-trnT-GGU	4	CGTAGTACTCTACCCCCAGG	CCGATGACTTACGCCTTACC	20	20	60.0	55.0	58.0	58.2	0.0	0.0	0.172449	33652	34566	1045-1050	1.000000	79.7
trnE-UUC-trnT-GGU_p5	trnE-UUC-trnT-GGU	5	GCTGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	20	20	55.0	50.0	58.3	58.0	0.0	0.0	0.269359	33652	34566	1013-1018	1.000000	78.9
trnT-GGU-psbD_p1	trnT-GGU-psbD	1	GTGGTAGAGTAACGCCATGG	ACAAAAACGAAACGGTCCCT	20	20	55.0	45.0	58.1	58.2	7.6	0.0	0.310137	34639	36155	1667-1667	1.000000	78.3
trnT-GGU-psbD_p2	trnT-GGU-psbD	2	GTGGTAGAGTAACGCCATGG	GACCGGACCAACCTACAAAA	20	20	55.0	50.0	58.1	57.7	7.6	9.5	0.323334	34639	36155	1681-1681	1.000000	78.2
trnT-GGU-psbD_p3	trnT-GGU-psbD	3	TCAGTGGTAGAGTAACGCCA	ACAAAAACGAAACGGTCCCT	20	20	50.0	45.0	58.1	58.2	0.0	0.0	0.332292	34639	36155	1670-1670	1.000000	78.2
trnT-GGU-psbD_p4	trnT-GGU-psbD	4	TCAGTGGTAGAGTAACGCCA	GACCGGACCAACCTACAAAA	20	20	50.0	50.0	58.1	57.7	0.0	9.5	0.345488	34639	36155	1684-1684	1.000000	78.0
trnT-GGU-psbD_p5	trnT-GGU-psbD	5	GTGGTAGAGTAACGCCATGG	TAGACCGGACCAACCTACAA	20	20	55.0	50.0	58.1	57.7	7.6	9.5	0.371563	34639	36155	1683-1683	1.000000	77.8
psaA_p1	psaA	1	AGCTAAGCCTTGGCTAAACC	GCGGGTCTCTTTGTATGTGT	20	20	50.0	50.0	57.9	57.9	14.2	0.0	0.225034	43086	45338	2359-2359	1.000000	78.3
psaA_p2	psaA	2	GCTAAGCCTTGGCTAAACCT	GCGGGTCTCTTTGTATGTGT	20	20	50.0	50.0	57.9	57.9	15.1	0.0	0.225034	43086	45338	2358-2358	1.000000	78.3
psaA_p3	psaA	3	AATACGACGAGTAGTGGGGT	GCGGGTCTCTTTGTATGTGT	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.229551	43086	45338	2383-2383	1.000000	78.3
psaA_p4	psaA	4	GTAGTGGGGTCCTGAGCTAA	GCGGGTCTCTTTGTATGTGT	20	20	55.0	50.0	58.1	57.9	0.0	0.0	0.232780	43086	45338	2373-2373	1.000000	78.2
psaA_p5	psaA	5	GCTAAGCCTTGGCTAAACCT	TTGGCGGGTCTCTTTGTATG	20	20	50.0	50.0	57.9	57.9	15.1	0.0	0.238222	43086	45338	2361-2361	1.000000	78.2
rps4_p1	rps4	1	GAGGCCAAAATTGTCCGAAC	ACTTTACTGGATTTCGCTTCA	20	21	50.0	38.1	57.7	55.5	5.0	0.0	3.815822	48867	49472	731-731	1.000000	49.8
rps4_p2	rps4	2	GGAGGCCAAAATTGTCCGAA	ACTTTACTGGATTTCGCTTCA	20	21	50.0	38.1	58.7	55.5	5.0	0.0	4.215025	48867	49472	732-732	1.000000	46.6
rps4_p3	rps4	3	GAGGCCAAAATTGTCCGAAC	ACTTTACTGGATTTCGCTTCAT	20	22	50.0	36.4	57.7	56.1	5.0	0.0	4.270533	48867	49472	731-731	1.000000	46.2
rps4_p4	rps4	4	CCAAAATTGTCCGAACCCTTG	ACTTTACTGGATTTCGCTTCA	21	21	47.6	38.1	58.0	55.5	0.0	0.0	4.493412	48867	49472	727-727	1.000000	44.4
rps4_p5	rps4	5	GCCAAAATTGTCCGAACCCT	ACTTTACTGGATTTCGCTTCA	20	21	50.0	38.1	59.0	55.5	0.0	0.0	4.501946	48867	49472	728-728	1.000000	44.3
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCGATTTC	20	20	55.0	55.0	57.9	58.2	0.0	10.2	0.381189	49896	50549	604-716	1.000000	81.4
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	GGAATCGAACCGATGACCAT	CCGTAGCGTCTACCGATTTC	20	20	50.0	55.0	57.8	58.2	15.5	10.2	0.488942	49896	50549	636-748	1.000000	80.5
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GCGATGCTCTAACCTCTGAG	CGTCTACCGATTTCGCCATA	20	20	55.0	50.0	57.9	57.6	0.0	0.0	0.543526	49896	50549	598-710	1.000000	80.1
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	GCGATGCTCTAACCTCTGAG	GGTTTCCATACCAAGGCTCA	20	20	55.0	50.0	57.9	57.5	0.0	0.0	0.640147	49896	50549	636-748	1.000000	79.3
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	GGAATCGAACCGATGACCAT	CGTCTACCGATTTCGCCATA	20	20	50.0	50.0	57.8	57.6	15.5	0.0	0.651279	49896	50549	630-742	1.000000	79.2
trnF-GAA-ndhJ_p1	trnF-GAA-ndhJ	1	CCTCGTGTCACCAGTTCAAA	CCTGAAAGTTGGGTGGGATG	20	20	50.0	55.0	58.1	58.5	0.0	0.0	0.502692	51522	52249	736-840	1.000000	80.3
trnF-GAA-ndhJ_p2	trnF-GAA-ndhJ	2	CCTCGTGTCACCAGTTCAAA	CTGAAAGTTGGGTGGGATGG	20	20	50.0	55.0	58.1	58.5	0.0	0.0	0.502692	51522	52249	735-839	1.000000	80.3
trnF-GAA-ndhJ_p3	trnF-GAA-ndhJ	3	CCTCGTGTCACCAGTTCAAA	TAATCATCCCCGCCTAAAGC	20	20	50.0	50.0	58.1	57.4	0.0	0.0	0.613374	51522	52249	767-871	1.000000	79.4
trnF-GAA-ndhJ_p4	trnF-GAA-ndhJ	4	CCTCGTGTCACCAGTTCAAA	AATGCCTGAAAGTTGGGTGG	20	20	50.0	50.0	58.1	58.7	0.0	0.0	0.710815	51522	52249	740-844	1.000000	78.6
trnF-GAA-ndhJ_p5	trnF-GAA-ndhJ	5	TCGGGATAGCTCAGTTGGTA	CTGAAAGTTGGGTGGGATGG	20	20	50.0	55.0	57.6	58.5	0.0	0.0	0.898205	51522	52249	773-877	1.000000	77.1
rpl16_p1	rpl16	1	CTCAAACAAAGACCGCTTTCC	AGACTAAGAAATGACAAATCGGTC	21	24	47.6	37.5	58.0	57.0	0.0	0.0	5.969910	83516	85007	1625-1627	1.000000	40.5
rpl16_p2	rpl16	2	CTCAAACAAAGACCGCTTTCC	GACTAAGAAATGACAAATCGGTCT	21	24	47.6	37.5	58.0	57.0	0.0	0.0	5.969910	83516	85007	1624-1626	1.000000	40.5
rpl16_p3	rpl16	3	CTCAAACAAAGACCGCTTTCC	ACTAAGAAATGACAAATCGGTCT	21	23	47.6	34.8	58.0	55.9	0.0	0.0	6.054825	83516	85007	1623-1625	1.000000	40.5
rpl16_p4	rpl16	4	CTCAAACAAAGACCGCTTTCC	AGACTAAGAAATGACAAATCGGT	21	23	47.6	34.8	58.0	55.9	0.0	0.0	6.054825	83516	85007	1625-1627	1.000000	40.5
rpl16_p5	rpl16	5	TCATCTCAAACAAAGACCGCT	GACTAAGAAATGACAAATCGGTCT	21	24	42.9	37.5	57.9	57.0	0.0	0.0	6.095829	83516	85007	1628-1630	1.000000	40.5
trnR-ACG-trnN-GUU_p1	trnR-ACG-trnN-GUU	1	CAGAGGATTAGAGCACGTGG	CCGTTGTTCCTCAGTAGCTC	20	20	55.0	55.0	57.8	58.0	18.9	0.0	0.218238	110676	111292	759-759	1.000000	78.8
trnR-ACG-trnN-GUU_p2	trnR-ACG-trnN-GUU	2	GAACAGATCCAGTGGAGACG	CCGTTGTTCCTCAGTAGCTC	20	20	55.0	55.0	57.7	58.0	18.7	0.0	0.288158	110676	111292	796-796	1.000000	78.2
trnR-ACG-trnN-GUU_p3	trnR-ACG-trnN-GUU	3	CAGAGGATTAGAGCACGTGG	TGGTCGTAGGTTCGAATCCT	20	20	55.0	50.0	57.8	58.2	18.9	18.9	0.372539	110676	111292	710-710	1.000000	77.6
trnR-ACG-trnN-GUU_p4	trnR-ACG-trnN-GUU	4	CAGAGGATTAGAGCACGTGG	CTGGTCGTAGGTTCGAATCC	20	20	55.0	55.0	57.8	57.8	18.9	4.4	0.421214	110676	111292	711-711	1.000000	77.2
trnR-ACG-trnN-GUU_p5	trnR-ACG-trnN-GUU	5	GAACAGATCCAGTGGAGACG	TGGTCGTAGGTTCGAATCCT	20	20	55.0	50.0	57.7	58.2	18.7	18.9	0.442458	110676	111292	747-747	1.000000	77.0
ndhF-rpl32_p1	ndhF-rpl32	1	TGTTGCCGTCGGAAAAAGTA	AAAAGCTTTCAACGCTGCTC	20	20	45.0	45.0	58.0	57.9	17.5	13.1	0.174633	115117	115753	829-832	1.000000	79.7
ndhF-rpl32_p2	ndhF-rpl32	2	TGTTGCCGTCGGAAAAAGTA	CGCCCTTTGCTTTTCCAAAT	20	20	45.0	45.0	58.0	57.8	17.5	0.0	0.217498	115117	115753	807-810	1.000000	79.3
ndhF-rpl32_p3	ndhF-rpl32	3	TTTGTTGCCGTCGGAAAAAG	AAAAGCTTTCAACGCTGCTC	20	20	45.0	45.0	57.8	57.9	17.5	13.1	0.321756	115117	115753	831-834	1.000000	78.5
ndhF-rpl32_p4	ndhF-rpl32	4	TTTGTTGCCGTCGGAAAAAG	CGCCCTTTGCTTTTCCAAAT	20	20	45.0	45.0	57.8	57.8	17.5	0.0	0.364621	115117	115753	809-812	1.000000	78.2
ndhF-rpl32_p5	ndhF-rpl32	5	TGTTGCCGTCGGAAAAAGTA	GCGAAAAAGCTTTCAACGCT	20	20	45.0	45.0	58.0	58.5	17.5	30.9	0.572067	115117	115753	833-836	1.000000	76.5
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	GAGCAGCGTTGAAAGCTTTT	AGCGTGTCTACCGATTTCAC	20	20	45.0	50.0	57.9	58.0	14.0	0.0	0.133292	115916	117101	1301-1302	1.000000	80.2
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	GAGCAGCGTTGAAAGCTTTT	CAGCGTGTCTACCGATTTCA	20	20	45.0	50.0	57.9	58.0	14.0	0.0	0.134662	115916	117101	1302-1303	1.000000	80.2
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	GAGCAGCGTTGAAAGCTTTT	CCCTTTTCTGACGAAGCGTA	20	20	45.0	50.0	57.9	57.9	14.0	0.0	0.200008	115916	117101	1597-1600	1.000000	79.8
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	GAGCAGCGTTGAAAGCTTTT	AATACAAAATGCCATGCCGC	20	20	45.0	45.0	57.9	58.1	14.0	0.0	0.186836	115916	117101	1366-1367	1.000000	79.7
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	GAGCAGCGTTGAAAGCTTTT	GGGGACTAAGAGTGGGGATT	20	20	45.0	55.0	57.9	58.1	14.0	0.0	0.230762	115916	117101	1859-1862	1.000000	79.5
ccsA_p1	ccsA	1	GTGAAATCGGTAGACACGCT	CAGTTGACAAGGTCGAAGCT	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.136750	117298	118269	1358-1361	1.000000	79.7
ccsA_p2	ccsA	2	TGAAATCGGTAGACACGCTG	CAGTTGACAAGGTCGAAGCT	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.138121	117298	118269	1357-1360	1.000000	79.7
ccsA_p3	ccsA	3	GTGAAATCGGTAGACACGCT	TCAGTTGACAAGGTCGAAGC	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.141965	117298	118269	1359-1362	1.000000	79.7
ccsA_p4	ccsA	4	TGAAATCGGTAGACACGCTG	TCAGTTGACAAGGTCGAAGC	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.143336	117298	118269	1358-1361	1.000000	79.7
ccsA_p5	ccsA	5	GAGAAAGCCGCTATGGTGAA	CAGTTGACAAGGTCGAAGCT	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.153095	117298	118269	1373-1376	1.000000	79.6
ycf1_p1	ycf1	1	TCGATCTTCATCTTCTGACT	TCTAGTGAGAACGTTTGGACA	20	21	40.0	42.9	53.2	56.6	0.0	0.5	7.139852	130581	130700	246-246	1.000000	42.3
ycf1_p2	ycf1	2	TCGATCTTCATCTTCTGACTT	TCTAGTGAGAACGTTTGGACA	21	21	38.1	42.9	54.1	56.6	0.0	0.5	7.234794	130581	130700	246-246	1.000000	42.3
ycf1_p3	ycf1	3	TTCGATCTTCATCTTCTGACT	TCTAGTGAGAACGTTTGGACA	21	21	38.1	42.9	54.1	56.6	0.0	0.5	7.234794	130581	130700	247-247	1.000000	42.3
ycf1_p4	ycf1	4	TCGATCTTCATCTTCTGACT	AGTGAGAACGTTTGGACAAA	20	20	40.0	40.0	53.2	55.4	0.0	0.5	7.326517	130581	130700	243-243	1.000000	42.3
ycf1_p5	ycf1	5	TTCGATCTTCATCTTCTGACTT	TCTAGTGAGAACGTTTGGACA	22	21	36.4	42.9	55.0	56.6	0.0	0.5	7.411544	130581	130700	247-247	1.000000	42.3
trnN-GUU-trnR-ACG_p1	trnN-GUU-trnR-ACG	1	CCGTTGTTCCTCAGTAGCTC	CAGAGGATTAGAGCACGTGG	20	20	55.0	55.0	58.0	57.8	0.0	18.9	0.218238	132747	133363	759-759	1.000000	78.8
trnN-GUU-trnR-ACG_p2	trnN-GUU-trnR-ACG	2	CCGTTGTTCCTCAGTAGCTC	GAACAGATCCAGTGGAGACG	20	20	55.0	55.0	58.0	57.7	0.0	18.7	0.288158	132747	133363	796-796	1.000000	78.2
trnN-GUU-trnR-ACG_p3	trnN-GUU-trnR-ACG	3	TGGTCGTAGGTTCGAATCCT	CAGAGGATTAGAGCACGTGG	20	20	50.0	55.0	58.2	57.8	18.9	18.9	0.372539	132747	133363	710-710	1.000000	77.6
trnN-GUU-trnR-ACG_p4	trnN-GUU-trnR-ACG	4	CTGGTCGTAGGTTCGAATCC	CAGAGGATTAGAGCACGTGG	20	20	55.0	55.0	57.8	57.8	4.4	18.9	0.421214	132747	133363	711-711	1.000000	77.2
trnN-GUU-trnR-ACG_p5	trnN-GUU-trnR-ACG	5	TGGTCGTAGGTTCGAATCCT	GAACAGATCCAGTGGAGACG	20	20	50.0	55.0	58.2	57.7	18.9	18.7	0.442458	132747	133363	747-747	1.000000	77.0