Hotspot

ycf1

SSC · 122056-127443 · 5388 bp

Back to Hemipilia

Pi 0.0166

MarkerSeek score 53.10

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5388
variable_sites: 134
pi: 0.0166
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGTGATTTACGCATTAATCGA	CCCGTTCTAATTCGACCGAT	33.3	50.0	53.8	57.5	227 bp	1.0	43.2
ycf1_p2	TGTGATTTACGCATTAATCGA	ATCGCCCGTTCTAATTCGAC	33.3	50.0	53.8	58.2	231 bp	1.0	43.1
ycf1_p3	TGTGATTTACGCATTAATCGAA	ATCGCCCGTTCTAATTCGAC	31.8	50.0	54.6	58.2	231 bp	1.0	43.1
ycf1_p4	ATGTGATTTACGCATTAATCGA	ATCGCCCGTTCTAATTCGAC	31.8	50.0	54.4	58.2	232 bp	1.0	43.1
ycf1_p5	TGTGATTTACGCATTAATCGA	AATCGCCCGTTCTAATTCGA	33.3	45.0	53.8	57.4	232 bp	1.0	43.1