Hotspot

ndhF-rpl32

SSC · 110917-111657 · 741 bp

Back to Hemipilia

Pi 0.0405

MarkerSeek score 75.20

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 741
variable_sites: 45
pi: 0.0405
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	CATTCCCACGAGTTCCCAAA	TCCACCGGACAGTCAAAAAG	50.0	50.0	58.1	58.0	1203-1272 bp	1.0	86.3
ndhF-rpl32_p2	AACACATTCCCACGAGTTCC	TCCACCGGACAGTCAAAAAG	50.0	50.0	58.1	58.0	1207-1276 bp	1.0	86.2
ndhF-rpl32_p3	GGTCGTGTGAACCAAAAACC	TCCACCGGACAGTCAAAAAG	50.0	50.0	57.8	58.0	1241-1310 bp	1.0	85.4
ndhF-rpl32_p4	GGGGGAATTGTGCGGATTTA	TCCACCGGACAGTCAAAAAG	50.0	50.0	58.2	58.0	1471-1540 bp	1.0	84.8
ndhF-rpl32_p5	CCACACATGAAGGGGGAATT	TCCACCGGACAGTCAAAAAG	50.0	50.0	57.8	58.0	1482-1551 bp	1.0	84.7