Hotspot

rps3-rpl22

LSC · 81898-81953 · 56 bp

Back to Hemipilia

Pi 0.0476

MarkerSeek score 62.20

Primer available yes

Label rps3-rpl22

Feature Metadata

label_name: rps3-rpl22
feature_type: igs
region: LSC
length_bp: 56
variable_sites: 4
pi: 0.0476
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3-rpl22_p1	TGAGTCTCCGGTGAAACTCT	CTTTCGTGAGACATGCGAGA	50.0	50.0	58.0	58.0	1006-1012 bp	1.0	82.7
rps3-rpl22_p2	TGAGTCTCCGGTGAAACTCT	TTATCATTCATTGGCGGGGG	50.0	50.0	58.0	58.0	804-809 bp	1.0	82.3
rps3-rpl22_p3	TGAGTCTCCGGTGAAACTCT	TGATCAGATTCGCGAACGTT	50.0	45.0	58.0	58.0	670-675 bp	1.0	82.2
rps3-rpl22_p4	TGAGTCTCCGGTGAAACTCT	TCAGATTCGCGAACGTTCTT	50.0	45.0	58.0	57.9	667-672 bp	1.0	81.8
rps3-rpl22_p5	TGAGTCTCCGGTGAAACTCT	TTGATCAGATTCGCGAACGT	50.0	45.0	58.0	58.0	671 bp	0.666667	66.6