Hotspot

psbB-psbT

LSC · 71760-72215 · 456 bp

Back to Hemipilia

Pi 0.0154

MarkerSeek score 63.20

Primer available yes

Label psbB-psbT

Feature Metadata

label_name: psbB-psbT
feature_type: igs
region: LSC
length_bp: 456
variable_sites: 10
pi: 0.0154
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbB-psbT_p1	GTGCCAGAACCTTGTTCAGA	TGGAACTTTAGGTGGTTCTCG	50.0	47.6	58.0	57.6	656-665 bp	1.0	73.8
psbB-psbT_p2	TGCCAGAACCTTGTTCAGAG	TGGAACTTTAGGTGGTTCTCG	50.0	47.6	57.7	57.6	655-664 bp	1.0	72.0
psbB-psbT_p3	TCAGAGATGTTTTTGCCGGT	TGGAACTTTAGGTGGTTCTCG	45.0	47.6	57.7	57.6	641-650 bp	1.0	71.9
psbB-psbT_p4	GTGCCAGAACCTTGTTCAGA	GGAACTTTAGGTGGTTCTCGA	50.0	47.6	58.0	57.3	655-664 bp	1.0	71.6
psbB-psbT_p5	TGCCAGAACCTTGTTCAGAG	GGAACTTTAGGTGGTTCTCGA	50.0	47.6	57.7	57.3	654-663 bp	1.0	69.7