Hotspot

atpF-atpH

LSC · 12453-12557 · 105 bp

Back to Hemipilia

Pi 0.0349

MarkerSeek score 62.80

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 105
variable_sites: 5
pi: 0.0349
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	CCGCGGAAACAAAAGAATCG	GGTCGTGGCATTAGCTCTTT	50.0	50.0	58.4	58.3	182-183 bp	1.0	77.4
atpF-atpH_p2	CGCGGAAACAAAAGAATCGG	GGTCGTGGCATTAGCTCTTT	50.0	50.0	58.4	58.3	181-182 bp	1.0	77.4
atpF-atpH_p3	GCGGAAACAAAAGAATCGGT	GGTCGTGGCATTAGCTCTTT	45.0	50.0	57.3	58.3	180-181 bp	1.0	75.0
atpF-atpH_p4	GCGGAAACAAAAGAATCGGTT	GGTCGTGGCATTAGCTCTTT	42.9	50.0	58.0	58.3	180-181 bp	1.0	72.5
atpF-atpH_p5	CGCGGAAACAAAAGAATCGG	TGGTCGTGGCATTAGCTCTT	50.0	50.0	58.4	59.4	182-183 bp	1.0	68.4