Hotspot

psbI-trnS-GCU

LSC · 7605-7735 · 131 bp

Back to Hemipilia

Pi 0.056

MarkerSeek score 60.50

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: LSC
length_bp: 131
variable_sites: 11
pi: 0.056
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	ATCTAATGATCCGGGGCGTA	GGGTTCGAATCCCTCTCTCT	50.0	55.0	58.1	58.2	194-210 bp	1.0	82.1
psbI-trnS-GCU_p2	TATCTAATGATCCGGGGCGT	GGGTTCGAATCCCTCTCTCT	50.0	55.0	58.1	58.2	195-211 bp	1.0	82.1
psbI-trnS-GCU_p3	TCTAATGATCCGGGGCGTAA	GGGTTCGAATCCCTCTCTCT	50.0	55.0	58.3	58.2	193-209 bp	1.0	80.4
psbI-trnS-GCU_p4	TAATGATCCGGGGCGTAATC	GGGTTCGAATCCCTCTCTCT	50.0	55.0	57.2	58.2	191-207 bp	1.0	76.7
psbI-trnS-GCU_p5	CTAATGATCCGGGGCGTAAT	GGGTTCGAATCCCTCTCTCT	50.0	55.0	57.2	58.2	192-208 bp	1.0	76.6