Hotspot

rps16-trnQ-UUG

LSC · 6054-6478 · 425 bp

Back to Hemipilia

Pi 0.0206
MarkerSeek score 62.40
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
425
variable_sites
12
pi
0.0206
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 GCACGTTGCTTTTTACCACA GAGGTTCGAATCCTTCCGTC 45.0 55.0 57.8 58.1 441-493 bp 1.0 83.8
rps16-trnQ-UUG_p2 TCATGTCCTTCAAGTCGCAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 457-509 bp 1.0 83.7
rps16-trnQ-UUG_p3 GCACGTTGCTTTTTACCACA CTCGGAGGTTCGAATCCTTC 45.0 55.0 57.8 57.8 445-497 bp 1.0 82.7
rps16-trnQ-UUG_p4 TCATGTCCTTCAAGTCGCAC CTCGGAGGTTCGAATCCTTC 50.0 55.0 58.2 57.8 461-513 bp 1.0 82.6
rps16-trnQ-UUG_p5 AGTCGCACGTTGCTTTTTAC GAGGTTCGAATCCTTCCGTC 45.0 55.0 57.6 58.1 445-497 bp 1.0 82.4