Hotspot

rpl16

LSC · 77445-78342 · 898 bp

Back to Hemionitis

Pi 0.156

MarkerSeek score 80.40

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 898
variable_sites: 138
pi: 0.156
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 80.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	CACTCCATTGCGTCGTCTTA	CAAATAGCCGGACGTCTGAA	50.0	50.0	58.0	58.0	2190 bp	0.5	59.9
rpl16_p2	CCCATCTCCACGTCTTTGAG	CAAATAGCCGGACGTCTGAA	55.0	50.0	58.0	58.0	1586 bp	0.5	59.8
rpl16_p3	CTCCCATCTCCACGTCTTTG	CAAATAGCCGGACGTCTGAA	55.0	50.0	58.0	58.0	1588 bp	0.5	59.8
rpl16_p4	CTCTTCAAGGACATGTCGGG	CAAATAGCCGGACGTCTGAA	55.0	50.0	58.0	58.0	2126 bp	0.5	59.8
rpl16_p5	CACTCCATTGCGTCGTCTTA	GCGTTGAATGGGCTAGAGAA	50.0	50.0	58.0	58.0	2153 bp	0.5	59.7