Hotspot

ndhF

SSC · 108990-111203 · 2214 bp

Back to Hemionitis

Pi 0.179

MarkerSeek score 78.20

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2214
variable_sites: 394
pi: 0.179
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	TGGAGCTTGGTGAAACGAAA	TGTGTAAGATTCGGCCCTTG	45.0	50.0	58.0	57.9	2759-2771 bp	1.0	87.3
ndhF_p2	TTGGAGCTTGGTGAAACGAA	TGTGTAAGATTCGGCCCTTG	45.0	50.0	58.0	57.9	2760-2772 bp	1.0	87.3
ndhF_p3	TAGGGAGAATGGGGAAAGCA	TGTGTAAGATTCGGCCCTTG	50.0	50.0	58.0	57.9	2840 bp	0.5	59.0
ndhF_p4	TAGGGAGAATGGGGAAAGCA	AACTGTGTAAGATTCGGCCC	50.0	50.0	58.0	57.9	2843 bp	0.5	59.0
ndhF_p5	TGGAGCTTGGTGAAACGAAA	AACTGTGTAAGATTCGGCCC	45.0	50.0	58.0	57.9	2774 bp	0.5	58.8