Hotspot

atpF

LSC · 11335-12621 · 1287 bp

Back to Hemionitis

Pi 0.102

MarkerSeek score 81.10

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1287
variable_sites: 129
pi: 0.102
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 81.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	AGTACGGTGCCGATGTTAAC	GCAAAACCTTCAAGGAGGGA	50.0	50.0	58.0	58.0	1491-1496 bp	1.0	89.3
atpF_p2	TTTGCTTGCGAATGATGCTG	GCAAAACCTTCAAGGAGGGA	45.0	50.0	58.0	58.0	1442-1447 bp	1.0	89.3
atpF_p3	AGTACGGTGCCGATGTTAAC	TTGCCGAAGTGGATAACTCG	50.0	50.0	58.0	58.0	1605 bp	0.5	60.0
atpF_p4	TTTGCTTGCGAATGATGCTG	TTGCCGAAGTGGATAACTCG	45.0	50.0	58.0	58.0	1556 bp	0.5	59.8
atpF_p5	AGTACGGTGCCGATGTTAAC	AAATCCGCCTGTCTAAGCTG	50.0	50.0	58.0	58.0	1887 bp	0.5	59.8