Hotspot

ccsA-ndhD

SSC · 114352-114751 · 400 bp

Back to Hemionitis

Pi 0.284

MarkerSeek score 78.80

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 400
variable_sites: 105
pi: 0.284
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 78.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	TACGGTGGGGTAGATAGCTG	TGGTTTGATTGGAGCTGCTT	55.0	45.0	58.0	58.0	1364 bp	0.5	59.9
ccsA-ndhD_p2	TACGGTGGGGTAGATAGCTG	TGTTGGTTGGAGCGATTCAA	55.0	45.0	58.0	58.0	1536 bp	0.5	59.7
ccsA-ndhD_p3	TACGGTGGGGTAGATAGCTG	ATCGGTTCCTTGACAGATGC	55.0	50.0	58.0	58.0	1420 bp	0.5	59.7
ccsA-ndhD_p4	TACGGTGGGGTAGATAGCTG	AGGGGCAGTTGGTACAGTAT	55.0	50.0	58.0	58.0	1115 bp	0.5	59.7
ccsA-ndhD_p5	TACGGTGGGGTAGATAGCTG	TTGGATCAGTTGGGAGGAGT	55.0	50.0	58.0	58.0	1297 bp	0.5	59.6