Hotspot

rpl14-rpl16

LSC · 76951-77444 · 494 bp

Back to Hemionitis

Pi 0.3

MarkerSeek score 79.60

Primer available yes

Label rpl14-rpl16

Feature Metadata

label_name: rpl14-rpl16
feature_type: igs
region: LSC
length_bp: 494
variable_sites: 131
pi: 0.3
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 79.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl14-rpl16_p1	ACTGTCGTGGAAATAGCGTC	CCTGCTTGGGTTACAGCTAG	50.0	55.0	58.0	58.0	1470-1507 bp	1.0	91.7
rpl14-rpl16_p2	CACTCCATTGCGTCGTCTTA	CCTGCTTGGGTTACAGCTAG	50.0	55.0	58.0	58.0	1019 bp	0.5	59.7
rpl14-rpl16_p3	ACTGTCGTGGAAATAGCGTC	GCTCAGGCTGCTATGTTGAT	50.0	50.0	58.0	58.0	1282 bp	0.5	59.7
rpl14-rpl16_p4	CACTCCATTGCGTCGTCTTA	GCTCAGGCTGCTATGTTGAT	50.0	50.0	58.0	58.0	794 bp	0.5	59.6
rpl14-rpl16_p5	CTCTTCAAGGACATGTCGGG	CCTGCTTGGGTTACAGCTAG	55.0	55.0	58.0	58.0	955 bp	0.5	59.6