Hotspot

rpl20-rps12

LSC · 64343-65288 · 946 bp

Back to Hemionitis

Pi 0.165

MarkerSeek score 80.50

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 946
variable_sites: 153
pi: 0.165
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 80.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	TCGAAAGCGAAATGGAGAGG	GGAAGCAGAAGAAGCATCGA	50.0	50.0	58.0	58.0	1897 bp	0.5	59.8
rpl20-rps12_p2	TCGTACAAGCATGGAGAAGC	GGAAGCAGAAGAAGCATCGA	50.0	50.0	58.0	58.0	1823 bp	0.5	59.8
rpl20-rps12_p3	CGTTCTGCACGTTTCTTTCC	GGAAGCAGAAGAAGCATCGA	50.0	50.0	58.0	58.0	1974 bp	0.5	59.7
rpl20-rps12_p4	TCGAAAGCGAAATGGAGAGG	GTAGTGGAACGAAATCCCCC	50.0	55.0	58.0	58.0	1424 bp	0.5	59.6
rpl20-rps12_p5	TCGTACAAGCATGGAGAAGC	GTAGTGGAACGAAATCCCCC	50.0	55.0	58.0	58.0	1350 bp	0.5	59.6