Hotspot

psbE-petL

LSC · 60830-61581 · 752 bp

Back to Hemionitis

Pi 0.216

MarkerSeek score 82.40

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 752
variable_sites: 146
pi: 0.216
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 82.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	GACTTCTTGTCGGCTCTCTG	CGGAATCGAGCCTAACACAA	55.0	50.0	58.0	58.0	1188-1250 bp	1.0	91.9
psbE-petL_p2	CCACGCTCTGTTTATTCGGA	CGGAATCGAGCCTAACACAA	50.0	50.0	58.0	58.0	1497 bp	0.5	59.9
psbE-petL_p3	TCCACGCTCTGTTTATTCGG	CGGAATCGAGCCTAACACAA	50.0	50.0	58.0	58.0	1498 bp	0.5	59.9
psbE-petL_p4	CCACGCTCTGTTTATTCGGA	TCGAATCCTATAGGGCGTGT	50.0	50.0	58.0	58.0	1817 bp	0.5	59.9
psbE-petL_p5	TCCACGCTCTGTTTATTCGG	TCGAATCCTATAGGGCGTGT	50.0	50.0	58.0	58.0	1818 bp	0.5	59.9