Hotspot

rps4-trnL-UAA

LSC · 44071-44815 · 745 bp

Back to Hemionitis

Pi 0.241

MarkerSeek score 84.50

Primer available yes

Label rps4-trnL-UAA

Feature Metadata

label_name: rps4-trnL-UAA
feature_type: igs
region: LSC
length_bp: 745
variable_sites: 175
pi: 0.241
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 84.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnL-UAA_p1	GCAAGGCCTGGCAAATTTTT	ACGTCTCCATACCTAGGCTC	45.0	55.0	58.4	58.0	840-846 bp	1.0	86.6
rps4-trnL-UAA_p2	GCAAGGCCTGGCAAATTTTT	CGTCTCCATACCTAGGCTCA	45.0	55.0	58.4	58.0	839-845 bp	1.0	86.6
rps4-trnL-UAA_p3	GCAAGGCCTGGCAAATTTTT	GCGTCTACCATTTCGCCATA	45.0	50.0	58.4	58.1	802-808 bp	1.0	85.7
rps4-trnL-UAA_p4	GGCCTGGCAAATTTTTCAGA	ACGTCTCCATACCTAGGCTC	45.0	55.0	57.2	58.0	836-842 bp	1.0	83.1
rps4-trnL-UAA_p5	GGCCTGGCAAATTTTTCAGA	CGTCTCCATACCTAGGCTCA	45.0	55.0	57.2	58.0	835-841 bp	1.0	83.1