Hotspot

trnS-GGA-rps4

LSC · 42991-43482 · 492 bp

Back to Hemionitis

Pi 0.259

MarkerSeek score 80.80

Primer available yes

Label trnS-GGA-rps4

Feature Metadata

label_name: trnS-GGA-rps4
feature_type: igs
region: LSC
length_bp: 492
variable_sites: 117
pi: 0.259
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 80.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GGA-rps4_p1	GCCAAATCGCCTCCTTTTTC	GTGCCGGATCATTTGACTCT	50.0	50.0	58.0	58.0	1145 bp	0.5	59.7
trnS-GGA-rps4_p2	GCCAAATCGCCTCCTTTTTC	CTCTCAATTTTGCGTGCGTT	50.0	45.0	58.0	58.0	1494 bp	0.5	59.7
trnS-GGA-rps4_p3	GCCAAATCGCCTCCTTTTTC	TGATGCGTCTAATTTGGGGG	50.0	50.0	58.0	57.9	1554 bp	0.5	59.6
trnS-GGA-rps4_p4	AAGCTGCGAAGGATAACTGG	GTGCCGGATCATTTGACTCT	50.0	50.0	58.0	58.0	892 bp	0.5	59.5
trnS-GGA-rps4_p5	TTCGAATCCCTCCCTTTCCT	GTGCCGGATCATTTGACTCT	50.0	50.0	58.0	58.0	650 bp	0.5	59.5