Hotspot

atpF-atpH

LSC · 12622-13023 · 402 bp

Back to Hemionitis

Pi 0.228
MarkerSeek score 81.10
Primer available yes
Label atpF-atpH

Feature Metadata

label_name
atpF-atpH
feature_type
igs
region
LSC
length_bp
402
variable_sites
87
pi
0.228
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.95
markerseek_score
81.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpF-atpH_p1 GACGATCTCACTTATACTTCTCGT TTGCAAATCCGCCTGTCTAA 41.7 45.0 57.7 57.8 446-451 bp 1.0 55.9
atpF-atpH_p2 GACGATCTCACTTATACTTCTCGT TTTGCAAATCCGCCTGTCTA 41.7 45.0 57.7 57.8 447-452 bp 1.0 55.9
atpF-atpH_p3 ACGATCTCACTTATACTTCTCGT TTGCAAATCCGCCTGTCTAA 39.1 45.0 56.7 57.8 445-450 bp 1.0 55.6
atpF-atpH_p4 ACGATCTCACTTATACTTCTCGT TTTGCAAATCCGCCTGTCTA 39.1 45.0 56.7 57.8 446-451 bp 1.0 55.6
atpF-atpH_p5 GACGATCTCACTTATACTTCTCGT ATTTGCAAATCCGCCTGTCT 41.7 45.0 57.7 58.5 448-453 bp 1.0 53.8