Hotspot

ycf1

SSC · 123563-129037 · 5475 bp

Back to Hemiboea

Pi 0.0122

MarkerSeek score 52.70

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5475
variable_sites: 338
pi: 0.0122
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 52.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CCTCAGACCATTCAGGAAGT	TGGGTAGGAAAAAGGAAAGT	50.0	40.0	56.5	54.0	268 bp	0.9375	45.3
ycf1_p2	TCCTCAGACCATTCAGGAAGT	TGGGTAGGAAAAAGGAAAGT	47.6	40.0	58.1	54.0	269 bp	0.9375	45.2
ycf1_p3	TCCTCAGACCATTCAGGAAGT	TTGGGTAGGAAAAAGGAAAGT	47.6	38.1	58.1	54.9	270 bp	0.9375	45.2
ycf1_p4	CCTCAGACCATTCAGGAAGT	TTGGGTAGGAAAAAGGAAAGT	50.0	38.1	56.5	54.9	269 bp	0.9375	45.2
ycf1_p5	TCCTCAGACCATTCAGGAAGT	ATTGGGTAGGAAAAAGGAAAGT	47.6	36.4	58.1	55.5	271 bp	0.9375	45.2