Hotspot

psbA

LSC · 279-1337 · 1059 bp

Back to Hemiboea

Pi 0.00253

MarkerSeek score 36.50

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1059
variable_sites: 14
pi: 0.00253
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 36.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	GATCCACTTGGCTACATCCG	GAAAGCCGTGTGCAATGAAA	55.0	45.0	58.1	57.9	1634-1655 bp	1.0	82.5
psbA_p2	CAATCCACTGCCTTGATCCA	GAAAGCCGTGTGCAATGAAA	50.0	45.0	57.9	57.9	1648-1669 bp	1.0	82.5
psbA_p3	AATCCACTGCCTTGATCCAC	GAAAGCCGTGTGCAATGAAA	50.0	45.0	57.9	57.9	1647-1668 bp	1.0	82.5
psbA_p4	ACAATCCACTGCCTTGATCC	GAAAGCCGTGTGCAATGAAA	50.0	45.0	57.9	57.9	1649-1670 bp	1.0	82.4
psbA_p5	CATCCGCCCCTCTACTATGA	GAAAGCCGTGTGCAATGAAA	55.0	45.0	58.1	57.9	1620-1636 bp	0.875	77.0