Hotspot

petD

LSC · 77291-78529 · 1239 bp

Back to Hemiboea

Pi 0.00753

MarkerSeek score 54.30

Primer available yes

Label petD

Feature Metadata

label_name: petD
feature_type: gene
region: LSC
length_bp: 1239
variable_sites: 33
pi: 0.00753
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD_p1	TCTCCGAAGAGAAAATGGAT	TCGCTTTGAAACGATTATTCCC	40.0	40.9	53.7	57.4	1283-1321 bp	1.0	45.1
petD_p2	TCTCCGAAGAGAAAATGGAT	CGCTTTGAAACGATTATTCCCT	40.0	40.9	53.7	57.4	1282-1320 bp	1.0	45.1
petD_p3	TCTCCGAAGAGAAAATGGATT	TCGCTTTGAAACGATTATTCCC	38.1	40.9	54.6	57.4	1283-1321 bp	1.0	45.1
petD_p4	TCTCCGAAGAGAAAATGGATT	CGCTTTGAAACGATTATTCCCT	38.1	40.9	54.6	57.4	1282-1320 bp	1.0	45.1
petD_p5	TCTCCGAAGAGAAAATGGAT	TTCCCTAGATACACATGCCT	40.0	45.0	53.7	55.3	1266-1304 bp	0.9375	42.1