Hotspot

ccsA-ndhD

SSC · 115168-115406 · 239 bp

Back to Hemiboea

Pi 0.0214

MarkerSeek score 69.00

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 239
variable_sites: 26
pi: 0.0214
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	AGCAATTGTGGCGTTTATTGG	ACCCGTACTAGGTATTGGTATGT	42.9	43.5	58.0	58.2	432-447 bp	1.0	55.7
ccsA-ndhD_p2	GCAATTGTGGCGTTTATTGGA	ACCCGTACTAGGTATTGGTATGT	42.9	43.5	58.0	58.2	431-446 bp	1.0	55.7
ccsA-ndhD_p3	AATTCAGCAATTGTGGCGTT	ACCCGTACTAGGTATTGGTATGT	40.0	43.5	57.2	58.2	450 bp	0.0625	10.4
ccsA-ndhD_p4	ATTCAGCAATTGTGGCGTTT	ACCCGTACTAGGTATTGGTATGT	40.0	43.5	57.2	58.2	449 bp	0.0625	10.4
ccsA-ndhD_p5	AATTCAGCAATTGTGGCGTTT	ACCCGTACTAGGTATTGGTATGT	38.1	43.5	57.9	58.2	450 bp	0.0625	8.1