Hotspot

ndhF-rpl32

SSC · 112264-112986 · 723 bp

Back to Hemiboea

Pi 0.0143

MarkerSeek score 63.90

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 723
variable_sites: 58
pi: 0.0143
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	AAAAGCCCACATACGACGAA	ACGATGCACAATACCCCTTC	45.0	50.0	57.8	58.0	852-978 bp	1.0	88.4
ndhF-rpl32_p2	GCAAGAGGTCGTGTAAACCA	ACGATGCACAATACCCCTTC	50.0	50.0	58.1	58.0	1238-1364 bp	1.0	87.7
ndhF-rpl32_p3	AATAGAGCAGCACACAACGT	ACGATGCACAATACCCCTTC	45.0	50.0	57.8	58.0	1424-1550 bp	1.0	87.0
ndhF-rpl32_p4	GCAGCACACAACGTAACAAA	ACGATGCACAATACCCCTTC	45.0	50.0	57.8	58.0	1418-1544 bp	1.0	86.9
ndhF-rpl32_p5	GCCCACATACGACGAAGATT	ACGATGCACAATACCCCTTC	50.0	50.0	58.1	58.0	848-955 bp	0.6875	75.5