Hotspot

psaA-ycf3

LSC · 42247-42972 · 726 bp

Back to Hemiboea

Pi 0.0144

MarkerSeek score 58.50

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 726
variable_sites: 39
pi: 0.0144
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 58.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GCATCAGCATGTAGGTTCCA	ATCAAGCCGCTGAGTATTGG	50.0	50.0	58.0	58.0	956-982 bp	1.0	86.7
psaA-ycf3_p2	GCATCAGCATGTAGGTTCCA	GGAAACAGGCTATAGCGCTT	50.0	50.0	58.0	58.0	938-964 bp	1.0	86.7
psaA-ycf3_p3	GCATCAGCATGTAGGTTCCA	TGGTAACGGGACACCAAAAG	50.0	50.0	58.0	58.0	1105-1131 bp	1.0	86.3
psaA-ycf3_p4	GTTATTCCAGATGCTCGCCA	ATCAAGCCGCTGAGTATTGG	50.0	50.0	58.0	58.0	1250-1276 bp	1.0	85.3
psaA-ycf3_p5	GTTATTCCAGATGCTCGCCA	TGGTAACGGGACACCAAAAG	50.0	50.0	58.0	58.0	1399-1425 bp	1.0	84.9