Hotspot

trnE-UUC-trnT-GGU

LSC · 31245-31928 · 684 bp

Back to Hemiboea

Pi 0.00795

MarkerSeek score 70.50

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 684
variable_sites: 32
pi: 0.00795
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 70.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	TTTCGTAGTACCCTACCCCC	GAACCGATGACTTACGCCTT	55.0	50.0	57.8	58.0	820-837 bp	1.0	85.3
trnE-UUC-trnT-GGU_p2	TTTCGTAGTACCCTACCCCC	CCATGGCGTTACTCTACCAC	55.0	55.0	57.8	58.1	799-816 bp	1.0	85.0
trnE-UUC-trnT-GGU_p3	TTTCGTAGTACCCTACCCCC	TGGCGTTACTCTACCACTGA	55.0	50.0	57.8	58.1	796-813 bp	1.0	84.9
trnE-UUC-trnT-GGU_p4	TTTCGTAGTACCCTACCCCC	CCGATGACTTACGCCTTACC	55.0	55.0	57.8	58.2	817-834 bp	1.0	84.2
trnE-UUC-trnT-GGU_p5	CTCCGTGAAAGAGAGGTGTC	GAACCGATGACTTACGCCTT	55.0	50.0	57.6	58.0	777-794 bp	1.0	84.1