Hotspot

trnK-UUU-rps16

LSC · 4147-4951 · 805 bp

Back to Hemiboea

Pi 0.0124

MarkerSeek score 70.60

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 805
variable_sites: 51
pi: 0.0124
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 70.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	GTCGGGGTCTTCCAAACTTT	TCTTGAAAGGGGTGCTCAAC	50.0	50.0	58.0	58.0	986-1023 bp	1.0	87.0
trnK-UUU-rps16_p2	AAAGCCGAGTACTCTACCGT	TCTTGAAAGGGGTGCTCAAC	50.0	50.0	58.2	58.0	904-941 bp	1.0	86.4
trnK-UUU-rps16_p3	AAGCCGAGTACTCTACCGTT	TCTTGAAAGGGGTGCTCAAC	50.0	50.0	58.2	58.0	903-940 bp	1.0	86.4
trnK-UUU-rps16_p4	GTCGGGGTCTTCCAAACTTT	GGTGCTCAACCTACAGGAAC	50.0	55.0	58.0	58.2	976-1013 bp	1.0	85.6
trnK-UUU-rps16_p5	TCTAGCCGCACTTAAAAGCC	TCTTGAAAGGGGTGCTCAAC	50.0	50.0	58.3	58.0	918-955 bp	1.0	85.6