Hotspot

rps3

LSC · 83497-84153 · 657 bp

Back to Hemerocallis

Pi 0.00101

MarkerSeek score 46.90

Primer available yes

Label rps3

Feature Metadata

label_name: rps3
feature_type: gene
region: LSC
length_bp: 657
variable_sites: 1
pi: 0.00101
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3_p1	GCGAGATAAATAACGAAAGGCC	AGACCTACTTGTCATATAACAATTGT	45.5	30.8	57.9	56.4	802 bp	1.0	40.1
rps3_p2	GGCGAGATAAATAACGAAAGGC	AGACCTACTTGTCATATAACAATTGT	45.5	30.8	57.9	56.4	803 bp	1.0	40.1
rps3_p3	GCGAGATAAATAACGAAAGGCC	GACCTACTTGTCATATAACAATTGT	45.5	32.0	57.9	55.2	801 bp	1.0	40.1
rps3_p4	GGCGAGATAAATAACGAAAGGC	GACCTACTTGTCATATAACAATTGT	45.5	32.0	57.9	55.2	802 bp	1.0	40.1
rps3_p5	GCGAGATAAATAACGAAAGGCC	AAGACCTACTTGTCATATAACAATTGT	45.5	29.6	57.9	57.0	803 bp	1.0	40.1