Hotspot

matK

LSC · 1687-3237 · 1551 bp

Back to Hemerocallis

Pi 0.00086

MarkerSeek score 46.70

Primer available yes

Label matK

Feature Metadata

label_name: matK
feature_type: gene
region: LSC
length_bp: 1551
variable_sites: 2
pi: 0.00086
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK_p1	CCACTACACTACTACATGTGCA	CCCTGTTTTGACCATATCGC	45.5	50.0	57.5	56.9	1749 bp	1.0	51.4
matK_p2	ACCACTACACTACTACATGTGC	CCCTGTTTTGACCATATCGC	45.5	50.0	57.5	56.9	1750 bp	1.0	51.4
matK_p3	CCACTACACTACTACATGTGCA	CCTGTTTTGACCATATCGCAC	45.5	47.6	57.5	57.6	1748 bp	1.0	49.2
matK_p4	ACCACTACACTACTACATGTGC	CCTGTTTTGACCATATCGCAC	45.5	47.6	57.5	57.6	1749 bp	1.0	49.2
matK_p5	CCACTACACTACTACATGTGCA	ACCCTGTTTTGACCATATCGC	45.5	47.6	57.5	58.6	1750 bp	1.0	47.4