Hotspot

clpP

LSC · 70411-72497 · 2087 bp

Back to Hemerocallis

Pi 0

MarkerSeek score 57.60

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2087
variable_sites: 0
pi: 0
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	GCATGCAATATCCAATCACGG	ACCCCATTCTTACGTTTCCA	47.6	45.0	58.1	56.7	2205-2207 bp	1.0	61.5
clpP_p2	GCATGCAATATCCAATCACGG	AACCCCATTCTTACGTTTCCA	47.6	42.9	58.1	57.5	2206-2208 bp	1.0	59.5
clpP_p3	GCATGCAATATCCAATCACGG	ACCCCATTCTTACGTTTCCAT	47.6	42.9	58.1	57.3	2205-2207 bp	1.0	57.7
clpP_p4	TGCATGCAATATCCAATCACG	ACCCCATTCTTACGTTTCCA	42.9	45.0	57.4	56.7	2206-2208 bp	1.0	57.0
clpP_p5	GCATGCAATATCCAATCACGG	AACCCCATTCTTACGTTTCCAT	47.6	40.9	58.1	58.0	2206-2208 bp	1.0	55.2