Hotspot

accD

LSC · 58290-59801 · 1512 bp

Back to Hemerocallis

Pi 0.000882

MarkerSeek score 59.30

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1512
variable_sites: 2
pi: 0.000882
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	GCGGGGCAGTAAGACTCT	TCCAACAAACAGGGATTCCT	61.1	45.0	58.7	56.6	1618 bp	1.0	47.5
accD_p2	GCGGGGCAGTAAGACTCT	ATCCAACAAACAGGGATTCCT	61.1	42.9	58.7	57.2	1619 bp	1.0	43.8
accD_p3	GCGGGGCAGTAAGACTCT	CCAACAAACAGGGATTCCTAGT	61.1	45.5	58.7	58.0	1617 bp	1.0	42.2
accD_p4	GCGGGGCAGTAAGACTCT	AATCCAACAAACAGGGATTCCT	61.1	40.9	58.7	57.9	1620 bp	1.0	41.4
accD_p5	GCGGGGCAGTAAGACTCT	CAATCCAACAAACAGGGATTCC	61.1	45.5	58.7	57.8	1621 bp	1.0	41.0