Hotspot

rpl32-ndhF

SSC · 126263-127294 · 1032 bp

Back to Hemerocallis

Pi 0.0042

MarkerSeek score 61.50

Primer available yes

Label rpl32-ndhF

Feature Metadata

label_name: rpl32-ndhF
feature_type: igs
region: SSC
length_bp: 1032
variable_sites: 6
pi: 0.0042
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-ndhF_p1	ACTGACTTTGCTAAAGAAAAAGCT	TGTTGCTGTCGGAATAAGCA	33.3	45.0	57.5	57.8	1244-1246 bp	1.0	43.4
rpl32-ndhF_p2	TGCAGCTAAATATCCCTTTTTCT	TGTTGCTGTCGGAATAAGCA	34.8	45.0	56.2	57.8	1215-1217 bp	1.0	40.8
rpl32-ndhF_p3	ACTGACTTTGCTAAAGAAAAAGC	TGTTGCTGTCGGAATAAGCA	34.8	45.0	56.2	57.8	1244-1246 bp	1.0	40.7
rpl32-ndhF_p4	ACTGACTTTGCTAAAGAAAAAGCT	CGGAATAAGCAGAAGTCCCA	33.3	50.0	57.5	57.3	1235-1237 bp	1.0	40.5
rpl32-ndhF_p5	GCTTTTATTGCAGCTAAATATCCCT	TGTTGCTGTCGGAATAAGCA	36.0	45.0	58.0	57.8	1223-1225 bp	1.0	40.5