Hotspot

trnL-UAG-rpl32

SSC · 125360-126082 · 723 bp

Back to Hemerocallis

Pi 0.0129

MarkerSeek score 67.00

Primer available yes

Label trnL-UAG-rpl32

Feature Metadata

label_name: trnL-UAG-rpl32
feature_type: igs
region: SSC
length_bp: 723
variable_sites: 14
pi: 0.0129
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-rpl32_p1	AGCGTGTCTACCGATTTCAC	AAGTCAGTTTCTACCGGGGA	50.0	50.0	58.0	58.0	827-829 bp	1.0	81.1
trnL-UAG-rpl32_p2	CAGCGTGTCTACCGATTTCA	AAGTCAGTTTCTACCGGGGA	50.0	50.0	58.0	58.0	828-830 bp	1.0	81.1
trnL-UAG-rpl32_p3	AGCGTGTCTACCGATTTCAC	AGTTTCTACCGGGGATTCCA	50.0	50.0	58.0	58.0	822-824 bp	1.0	81.0
trnL-UAG-rpl32_p4	CAGCGTGTCTACCGATTTCA	AGTTTCTACCGGGGATTCCA	50.0	50.0	58.0	58.0	823-825 bp	1.0	81.0
trnL-UAG-rpl32_p5	AGCGTGTCTACCGATTTCAC	TTTGTGCGACAACGACAAAC	50.0	45.0	58.0	58.1	794-796 bp	1.0	80.4