Hotspot

ndhD-ccsA

SSC · 123973-124633 · 661 bp

Back to Hemerocallis

Pi 0

MarkerSeek score 46.90

Primer available yes

Label ndhD-ccsA

Feature Metadata

label_name: ndhD-ccsA
feature_type: igs
region: SSC
length_bp: 661
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-ccsA_p1	CGCTCTGTATCTTTCTACCCG	GCAGCTCTTTTATGTGGATCA	52.4	42.9	58.0	56.3	886-893 bp	1.0	50.9
ndhD-ccsA_p2	CGCTCTGTATCTTTCTACCCG	GCAGCTCTTTTATGTGGATCAT	52.4	40.9	58.0	56.8	886-893 bp	1.0	47.0
ndhD-ccsA_p3	CGCTCTGTATCTTTCTACCCG	GCAGCTCTTTTATGTGGATCATT	52.4	39.1	58.0	57.5	886-893 bp	1.0	44.3
ndhD-ccsA_p4	CGCTCTGTATCTTTCTACCCG	GCAGCTCTTTTATGTGGATC	52.4	45.0	58.0	54.5	886-893 bp	1.0	44.1
ndhD-ccsA_p5	GCTCTGTATCTTTCTACCCGT	GCAGCTCTTTTATGTGGATCA	47.6	42.9	56.9	56.3	885-892 bp	1.0	42.3