Hotspot

trnT-UGU-trnL-UAA

LSC · 46789-47774 · 986 bp

Back to Hemerocallis

Pi 0.000677

MarkerSeek score 59.30

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 986
variable_sites: 1
pi: 0.000677
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	GCGATGCTCTAACCTCTGAG	ATCCAATCAAGTCCGTAGCG	55.0	50.0	57.9	57.8	1059-1284 bp	1.0	77.3
trnT-UGU-trnL-UAA_p2	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCGATTTC	55.0	55.0	57.9	58.2	1047-1272 bp	1.0	77.1
trnT-UGU-trnL-UAA_p3	GCGATGCTCTAACCTCTGAG	CAATCAAGTCCGTAGCGTCT	55.0	50.0	57.9	57.7	1056-1281 bp	1.0	76.8
trnT-UGU-trnL-UAA_p4	GGAATCGAACCGATGACCAT	ATCCAATCAAGTCCGTAGCG	50.0	50.0	57.8	57.8	1091-1316 bp	1.0	76.4
trnT-UGU-trnL-UAA_p5	GGAATCGAACCGATGACCAT	CCGTAGCGTCTACCGATTTC	50.0	55.0	57.8	58.2	1079-1304 bp	1.0	76.2