Hotspot

rpoB-trnC-GCA

LSC · 27864-28939 · 1076 bp

Back to Hemerocallis

Pi 0.00124

MarkerSeek score 59.60

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1076
variable_sites: 2
pi: 0.00124
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	AGGTATGGTGGACATTCCCT	TAAAGGATTTGCAGTCCCCC	50.0	50.0	57.7	57.5	1158-1161 bp	1.0	73.7
rpoB-trnC-GCA_p2	CAGGTATGGTGGACATTCCC	TAAAGGATTTGCAGTCCCCC	55.0	50.0	57.7	57.5	1159-1162 bp	1.0	73.2
rpoB-trnC-GCA_p3	CCAGGTATGGTGGACATTCC	TAAAGGATTTGCAGTCCCCC	55.0	50.0	57.7	57.5	1160-1163 bp	1.0	73.2
rpoB-trnC-GCA_p4	AGGTATGGTGGACATTCCCT	TTTTTGAGAAGGCGACACCC	50.0	50.0	57.7	58.7	1194-1197 bp	1.0	72.4
rpoB-trnC-GCA_p5	CAGGTATGGTGGACATTCCC	TTTTTGAGAAGGCGACACCC	55.0	50.0	57.7	58.7	1195-1198 bp	1.0	71.9