Hotspot

ycf1

SSC · 127929-133466 · 5538 bp

Back to Helwingia

Pi 0.000843

MarkerSeek score 53.00

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5538
variable_sites: 7
pi: 0.000843
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ATGCTTGTTCTAGCGGTCAG	AACAGCCTCAAACGAAACAA	50.0	40.0	58.0	56.4	255 bp	1.0	68.3
ycf1_p2	ATGCTTGTTCTAGCGGTCAG	ACAGCCTCAAACGAAACAAA	50.0	40.0	58.0	56.4	254 bp	1.0	68.3
ycf1_p3	TGTTCTAGCGGTCAGATCCT	AACAGCCTCAAACGAAACAA	50.0	40.0	57.9	56.4	250 bp	1.0	67.3
ycf1_p4	TGTTCTAGCGGTCAGATCCT	ACAGCCTCAAACGAAACAAA	50.0	40.0	57.9	56.4	249 bp	1.0	67.3
ycf1_p5	ATGCTTGTTCTAGCGGTCAG	ACAGCCTCAAACGAAACAAAA	50.0	38.1	58.0	57.1	254 bp	1.0	66.3