Hotspot

trnD-GUC

LSC · 32238-32311 · 74 bp

Back to Helwingia

Pi 0.018
MarkerSeek score 51.50
Primer available yes
Label trnD-GUC

Feature Metadata

label_name
trnD-GUC
feature_type
tRNA
region
LSC
length_bp
74
variable_sites
2
pi
0.018
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
51.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnD-GUC_p1 AGTAAGGATCTCCCCTTGGG TTCGCCAATCTACCATGAGA 55.0 45.0 57.8 56.3 279 bp 1.0 65.7
trnD-GUC_p2 AGTAAGGATCTCCCCTTGGG TCGCCAATCTACCATGAGAT 55.0 45.0 57.8 56.1 278 bp 1.0 63.8
trnD-GUC_p3 TGAAATTCTGCTCCCTGTCC TTCGCCAATCTACCATGAGA 50.0 45.0 57.5 56.3 257 bp 1.0 63.3
trnD-GUC_p4 AGTAAGGATCTCCCCTTGGG CCAAGAATTCGCCAATCTACC 55.0 47.6 57.8 57.0 286 bp 1.0 63.2
trnD-GUC_p5 CCTGTCCTCCCTTTCACAGA TTCGCCAATCTACCATGAGA 55.0 45.0 58.6 56.3 244 bp 1.0 62.2