Hotspot

rps2

LSC · 16675-17385 · 711 bp

Back to Helwingia

Pi 0

MarkerSeek score 26.70

Primer available yes

Label rps2

Feature Metadata

label_name: rps2
feature_type: gene
region: LSC
length_bp: 711
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 26.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2_p1	AAGCCAAAGCAACAGTCGTA	TTACCCGGCGAACTAATTGG	45.0	50.0	58.0	58.0	1990-1991 bp	1.0	79.6
rps2_p2	ATGGACTTGGAAACCCCCTA	TTACCCGGCGAACTAATTGG	50.0	50.0	57.9	58.0	1674-1675 bp	1.0	79.4
rps2_p3	AAGCCAAAGCAACAGTCGTA	AATGTCTACGGCCAATCGAC	45.0	50.0	58.0	58.1	1455 bp	1.0	79.3
rps2_p4	ATGGACTTGGAAACCCCCTA	AATGTCTACGGCCAATCGAC	50.0	50.0	57.9	58.1	1139 bp	1.0	79.1
rps2_p5	AAGCCAAAGCAACAGTCGTA	AGGCCTGAAAGAGAACGTTG	45.0	50.0	58.0	58.1	1790-1791 bp	1.0	78.9