Hotspot

rpoC2

LSC · 17607-21758 · 4152 bp

Back to Helwingia

Pi 0.000963

MarkerSeek score 46.90

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: LSC
length_bp: 4152
variable_sites: 6
pi: 0.000963
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	GCGAAGGGGAAACTAGACTG	AGTGATTGCTGAGATTCGCG	55.0	50.0	58.0	58.7	218 bp	1.0	74.9
rpoC2_p2	AGCGAAGGGGAAACTAGACT	AGTGATTGCTGAGATTCGCG	50.0	50.0	58.1	58.7	219 bp	1.0	74.5
rpoC2_p3	CTAGACTGGATCTGCATGGC	AGTGATTGCTGAGATTCGCG	55.0	50.0	57.8	58.7	206 bp	1.0	73.7
rpoC2_p4	TAGACTGGATCTGCATGGCT	AGTGATTGCTGAGATTCGCG	50.0	50.0	58.2	58.7	205 bp	1.0	73.4
rpoC2_p5	GAGCGAAGGGGAAACTAGAC	AGTGATTGCTGAGATTCGCG	55.0	50.0	57.7	58.7	220 bp	1.0	72.6