Hotspot

petL-petG

LSC · 69189-69362 · 174 bp

Back to Helwingia

Pi 0.046

MarkerSeek score 54.40

Primer available yes

Label petL-petG

Feature Metadata

label_name: petL-petG
feature_type: igs
region: LSC
length_bp: 174
variable_sites: 12
pi: 0.046
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petL-petG_p1	TTCGGTTTTCTACTAGCGGC	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	58.0	58.0	568-571 bp	1.0	83.8
petL-petG_p2	CGGTTTTCTACTAGCGGCTT	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	58.0	58.0	566-569 bp	1.0	83.8
petL-petG_p3	TTCGGTTTTCTACTAGCGGC	AGAACGTGGGTCTCCAAAAC	50.0	50.0	58.0	58.0	546-549 bp	1.0	83.8
petL-petG_p4	TTCGGTTTTCTACTAGCGGC	GGATTCCGTTCCCGATTTCA	50.0	50.0	58.0	58.0	973-976 bp	1.0	82.1
petL-petG_p5	TTCGGTTTTCTACTAGCGGC	TGGATTCCGTTCCCGATTTC	50.0	50.0	58.0	58.0	974-977 bp	1.0	82.1