Hotspot

trnF-GAA-ndhJ

LSC · 51061-51734 · 674 bp

Back to Helwingia

Pi 0.00198

MarkerSeek score 60.00

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 674
variable_sites: 2
pi: 0.00198
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	AGTTGGATAGGATGGCCCTT	55.0	50.0	58.0	58.1	816-821 bp	1.0	79.5
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	AGTTGGATAGGATGGCCCTT	50.0	50.0	58.1	58.1	780-785 bp	1.0	79.1
trnF-GAA-ndhJ_p3	GGGATAGCTCAGCTGGTAGA	ATCATCCACGCTTGAAACGT	55.0	45.0	58.0	58.2	851-856 bp	1.0	78.6
trnF-GAA-ndhJ_p4	CTGGTAGAGCAGAGGACTGA	AGTTGGATAGGATGGCCCTT	55.0	50.0	57.9	58.1	804-809 bp	1.0	78.4
trnF-GAA-ndhJ_p5	CCTCGTGTCACCAGTTCAAA	ATCATCCACGCTTGAAACGT	50.0	45.0	58.1	58.2	815-820 bp	1.0	78.2