Hotspot

ycf3-trnS-GGA

LSC · 46784-47664 · 881 bp

Back to Helwingia

Pi 0.000757

MarkerSeek score 59.50

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 881
variable_sites: 1
pi: 0.000757
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	TTCTCCTGAAGTTGTCGGAA	CCCTCGGTAAACAAAAGCCT	45.0	50.0	56.4	58.1	1042-1044 bp	1.0	66.8
ycf3-trnS-GGA_p2	TTTCTCCTGAAGTTGTCGGA	CCCTCGGTAAACAAAAGCCT	45.0	50.0	56.4	58.1	1043-1045 bp	1.0	66.8
ycf3-trnS-GGA_p3	TTCTCCTGAAGTTGTCGGAA	ACGGAAAGAGAGGGATTCGA	45.0	50.0	56.4	58.2	1063-1065 bp	1.0	66.3
ycf3-trnS-GGA_p4	TTTCTCCTGAAGTTGTCGGA	ACGGAAAGAGAGGGATTCGA	45.0	50.0	56.4	58.2	1064-1066 bp	1.0	66.3
ycf3-trnS-GGA_p5	TTCTCCTGAAGTTGTCGGAA	TTCCAATGCTACGCCTTGAA	45.0	45.0	56.4	57.8	1013-1015 bp	1.0	66.0