Hotspot

trnS-GCU-trnT-CGU

LSC · 9047-9776 · 730 bp

Back to Helwingia

Pi 0.000913

MarkerSeek score 59.50

Primer available yes

Label trnS-GCU-trnT-CGU

Feature Metadata

label_name: trnS-GCU-trnT-CGU
feature_type: igs
region: LSC
length_bp: 730
variable_sites: 1
pi: 0.000913
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnT-CGU_p1	ATTAGCAATCCGCCGCTTTA	GCAAACCGAAAGACCCCTTA	45.0	50.0	58.0	58.1	845-846 bp	1.0	79.1
trnS-GCU-trnT-CGU_p2	ACGGAAAGAGAGGGATTCGA	GCAAACCGAAAGACCCCTTA	50.0	50.0	58.2	58.1	894-895 bp	1.0	78.2
trnS-GCU-trnT-CGU_p3	CGAAAACCTCGTACAACGGA	GCAAACCGAAAGACCCCTTA	50.0	50.0	58.2	58.1	864-865 bp	1.0	77.5
trnS-GCU-trnT-CGU_p4	GCTTTAGTCCACTCAGCCAT	GCAAACCGAAAGACCCCTTA	50.0	50.0	57.6	58.1	831-832 bp	1.0	76.1
trnS-GCU-trnT-CGU_p5	AGTCCACTCAGCCATCTCTC	GCAAACCGAAAGACCCCTTA	55.0	50.0	58.5	58.1	826-827 bp	1.0	75.3